Collaborating researchers from CSGI (Lucia Paolini, Peter Nejsum, Annalisa Radeghieri, Nicolas Touzet, Marca H.M. Wauben, Antonella Bongiovanni and Paolo Bergese) published the paper “Large-scale production of extracellular vesicles: Report on the “massiveEVs” ISEV Workshop” in the Journal of Extracellular Biology (ISEV)
Friday 3rd of February 2023 Paolo Bergese gave an invited keynote lecture entitled “6 + 1 out-of-the-box EV problems” at ISEV workshop “QuantitatEVs” in Milan.
The ‘QuantitatEVs’ workshop aimed to discuss quantitative strategies and harmonized wet and computational approaches towards the comprehensive analysis of EVs. Ranging from bulk to single vesicle analyses and with a focus on emerging technologies, the workshop will covered the quantitative analysis of different EV-associated molecular components and relevant EV biophysical features which are the core of EV-associated biomarkers discovery and validation for their clinical translation.
evFOUNDRY article titled 'A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles' will be showcased in this collection celebrating excellent work led by female researchers across Nanoscale Horizons, Nanoscale and Nanoscale Advances.
Read the collection here: Celebrating International Womens' Day: Women in Nanoscience
The study concerns patients admitted to Intensive Care for sepsis, a condition of extreme severity that affects 50 million people worldwide every year. The project is, therefore, characterized by an explicit application orientation, with the ambition to provide information potentially useful to guide the choices of the National Health Service, patients and citizens. The research focuses on the identification of new nanostructured biomarkers of muscle weakness acquired in Intensive Care in patients with sepsis, To this end, extracellular vesicles will be isolated from the muscle of patients and characterized by a multidisciplinary approach that sees the combination of biochemistry, nanotechnologies and different -omics.
The research will be conducted, in synergy with the two Lombardy ASST, by two departments of the University of Brescia, the Department of Molecular and Translational Medicine (DMMT) and the Department of Medical-Surgical Specialties, Radiological Sciences and Public Health (DSMC). The operative team will be composed by evFOUNDRY researchers Dott.ssa Lucia Paolini, Prof. Paolo Bergese and Prof. Annalisa Radeghieri.
evFOUNDRY researchers Debora Berti, Marca Wauben and Paolo Bergese have been appreciated teachers at the XXVII SCHOOL OF PURE AND APPLIED BIOPHYSICS, this year dedicated to "Extracellular vesicles: from biophysical to translational challenges” and held in Venice on February 6-10 2023.
The school focussed on contemporary emerging scientific topics. The selected subjects have drawn young researchers' attention to frontier research issues of considerable scientific and educational impact. The School was held in the magnificent Palazzo Franchetti, the seat of the Istituto Veneto located in the historical centre of Venice. In 2023, the school focussed on Extracellular vesicles (EVs) covering the following topics:
(i) EVs colloidal and surface properties
(ii) techniques for biophysical characterization
(iii) EVs versus other (synthetic and extracellular) nanoparticles
(iv) EVs manipulation and engineering.
The 9th of September 2022, evFOUNDRY members Paolo Bergese and Lucia Paolini together with Miriam Romano, Rossella Zenatelli, Angelo Musicò, members of the FET-PROACTIVE project BOW (https://www.bowproject.eu/), from the University of Brescia and Dolores Di Vizio from Cedars-Sinai Medical Center and University of California Los Angeles gave an overview on the bio-nanotechnologies world and how they are applied to the medical field.
The open-to-the public event was supported by the association “Procida Coraggiosa”, Comune di Procida and the “62° Fiera del libro di Procida” in the framework of “Procida Italian capital of culture 2022” manifestation.
evFOUNDRY members Paolo Bergese, Lucia Paolini, Miriam Romano and Rossella Zenatelli will take part to the Bio-nanoscience scientific talk: "On the border between chemistry, physics and biology" that will take place in Procida on 7th September 2022.
The students of the State Higher Institute "F. Caracciolo - G. Da Procida" will participate too.
Nanotechnology is considered one of the driving forces of the next technological revolution. Bionanotechnology is the application in the biology and medicine of nanoscience and to be understood it is necessary that physics, chemistry and biology meet (re).
evFOUNDRY contributed to the 11th edition of the International Society for Extracellular Vesicles annual meeting (ISEV2022) in Lyon (France) 25-29 May 2022 ISEV 2022 with four oral presentations:
1. Detecting the collective nanomechanical fingerprint of EV populations by COlloidal NANoplasmonics (CONAN)
2. Pre-processing of bovine milk prior to EV isolation is essential for purity, but various protocols affect colloidal and functional properties of milk EVs
3. High-throughput nanomechanical differentiation of lipoproteins and extracellular vesicles
4. At which size does the membrane-to-lumen protein partition switch?
In addition, Marca Wauben chaired the oral sessions titled “EV detection”.
The 26th of April 2022 evFOUNDRY members met telematically together with the project officer Athina Zampara and the project reviewers for the final review meeting.
The project reviewers were: An Hendrix from the University of Ghent (The Netherlands), Irina Nazarenko form the University of Friburg (Germany), two experts in the EV field; Arben Mecoçi from Catalan Institute of Nanoscience and Nanotechnology (Spain), expert in nanobioelectronics & biosensors; Susana Castel, co-Founder and CEO at Frontwave Imaging, innovation radar expert.
evFOUNDRY consortium showed and discussed the progresses of the project and goals reached during 42 months of intense and collaborative work.
Collaborating researchers from Aarhus University (Anne Borup, Anders T. Boysen and Peter Nejsum) and CSGI (Andrea Ridolfi, Marco Brucale, Francesco Valle, Lucia Paolini and Paolo Bergese) published their results on Ascaris suum EVs. The authors compared three methods for EV separation to determine the most optimal strategy based on several characterisation methods and the immunomodulatory abilities of the helminth EVs.
evFOUNDRY article titled 'A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles' was one of the most popular articles published in Nanoscale Horizons last year, determined by the citations and page views. The paper is now part of the Themed collection “Nanoscale Horizons Most Popular 2021 Articles”
Read the collection here: https://pubs.rsc.org/en/Journals
For the 3rd year the Aarhus group members of evFOUNDRY organized the 3 day long course ‘Extracellular Vesicles (EVs) – an introduction’ at Aarhus University, Denmark.
The starting day is the 22nd of March. Aim of the course is to provide theoretical knowledge on EV, their role and potential in future therapeutics, and hands-on experience with EV isolation and characterization. The course is open to a wide audience including students with a university degree in medicine, dentistry, nursing, or master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).
A new contribution by evFOUNDRY researchers from evFOUNDRY researchers Lucrezia Caselli, Costanza Montis and Debora Berti (CSGI Florence), Andrea Ridolfi (CSGI Bologna), Tommy Nylander (University of Lund): “Interaction of nanoparticles with lipid films: the role of symmetry and shape anisotropy” has been published on the journal Physical Chemistry Chemical Physics (Phys. Chem. Chem. Phys., 2022,24, 2762-2776).
The study, unveiling a prominent role of symmetry at the nano-bio interfaces and, more in general, contributing to disentangle the function of non-lamellar structures in biological systems, is part is part of the themed collection 2021 PCCP HOT Articles and PCCP 2021 Emerging Investigators.
Martijn van Herwijnen from Utrecht University gave a presentation about the characterization of bovine milk extracellular vesicles at the annual meeting of the Netherlands Society of Extracellular Vesicles (NL-SEV). This results presented came from the collective efforts of the evFOUNDRY consortium and showed that the purity, and colloidal and functional properties of milk EVs are affected by the way EVs are purified.
evFOUNDRY member Anne Borup from Aarhus University attended the Danish Society for Extracellular Vesicles (DSEV) Annual Symposium 2021 held in Vejle, Denmark on the 28th of October. Anne Borup contributed with an oral presentation titled ‘Comparison of three separation methods for immunomodulatory extracellular vesicles from helminths’ for which she was awarded the ‘Best oral presentation’ by audience vote.”
evFOUNDRY project, in collaboration with the International Society of Extracellular Vesicles (ISEV) and FET-OPEN project VES4US, organized the international ISEV workshop massivEVs on large scale production of EVs https://www.isev.org/workshops
The event took place the 28th-29th of October 2021 at the Hotel Acquaviva del Garda in Desenzano del Garda (Brescia, Italy) with 50 participants in persons and 30 virtually connected.
evFOUNDRY members were part of the International Organizing Committee (Paolo Bergese co-chair with Antonella Bongiovanni, Marca Wauben, Peter Nejsum, Lucia Paolini, Annalisa Radeghieri) and have been selected for oral and poster presentations (Natasa Zarovni, Martijn van Herwijnen, Hywel Morgan, Anders Toftegaard Boysen).
Richard Gillams from Southampton University virtually attended the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021) from 10th to 14nd of October 2021.
He presented a poster entitled: “Nanoparticle and nano vesicle separation using electrokinetic biasedDeterministic Lateral Displacement micro channels”.
On September 28th-30th, 2021, Francesca Loria (evFOUNDRY member, HBM-LS) attended the Summer School “Valorization of Food Industry Wastes and By-products” held by the VALORTECH ERA Chair at the University of Life Sciences, Estonia. The educational programme aimed at offering an insightful training on the variety of food wastes and by-products that could be generated along the entire food supply chain, currently conceptualized food wastes and by-products management and valorization strategies, as well as the wide range of novel ´green´ processing technologies/techniques that could be adopted to obtain value-added bioactive components with high exploitable potential in the food, cosmetics and pharmaceutical industries.
To get further information on the VALORTECH Summer School program, please, visit the following webite: https://www.valortechevents.com/
Researchers from CSGI (Brescia unit) attended at the 2nd Symposium of the Italian Society of Extracellular Vesicles (EVIta) held in Barga (LU, Italy) from 20th to 22nd of September 2021.
Annalisa Radeghieri participated as member of the Organizing committee and Chair of two sessions:
and with the oral presentation entitled: “Specific antithrombin glycoforms adsorb onto plasma extracellular vesicles”.
Lucia Paolini presented:
1. Andrea Ridolfi (Oral presentation): The mechanical response of bicontinuous cubic phase lipid membranse to nanoscale deformations
2. Lucrezia Caselli (Key-note presentation): Assessing the stiffness of synthetic and natural nanovesicles by a “plasmon-based nanoruler”
3. Costanza Montis (Oral presentation): Challenging lamellar and non-lamellar lipid assemblies with nanoparticles: the role of symmetry at nano-bio interfaces
4. Debora Berti (Key-note presentation): Hybrid assemblies built from lipids an inorganic nanoparticles: from interaction to design of nanostructures devices
5. Tommy Nylander (Oral presentation): Morphologies and structure of brain lipid membrane dispersions
The 6 th and 7 th of July evFOUNDRY members met both in person and telematics for the month 35 partners meeting.
After more than a year, part of the evFOUNDRY consortium (CSGI and LUND) met in person at the University of Brescia for two collaborative days of discussion while all the other members joined in telematic mode.
This work represents a first step towards the identification of optimal serum inactivation protocols targeted to EV analysis. The Extracellular Vesicles (EVs) contained in the serum have been analyzed upon the application of the main practices used to inactivate SARS-CoV-2, the group of CSGI-Bologna in particular have applied the protocol recently developed to measured the changes induced in the EVs size and nano mechanical properties.
The CSGI team of evFOUNDRY developed a conceptual and methodological advancement which allows accessing the stiffness of extracellular vesicles, or more generally of membrane-enveloped nanosized objects, such as viruses. The measurement of this nanoscale property was achieved using a probe of a similar length scale, that is 10-nm gold nanoparticles which behave. Leveraging the plasmonic properties of these sensitive and fast nanoprobes, they set-up a very simple approach, with a straightforward colorimetric readout, easily accessible with basic lab equipment and expertise. Their discovery has been published and featured on the front cover of Nanoscale Horizons, which is a leading journal for the publication of urgent short reports of exceptionally high quality and innovative nanotechnology. These findings pave the way for a new field of investigation, where the stiffness of membranous nanoparticles can be related to biological properties, such as pharmacokinetics and cell internalization. The “plasmon-based stiffness nanoruler” is a reproducible, sensitive, high-throughput, and readily accessible method, which makes it possible the measurement of tiny amounts of sample.
Read more at: https://pubs.rsc.org/en/content/articlelanding/2021/nh/d1nh00012h#!divAbstract
This new research has been warmly welcomed by the colloids community and will be presented shortly at the international conferences of:
- 2021 ACS FALL NATIONAL MEETING, Atlanta, USA, 22-26 August 2021: https://www.agrodiv.org/event/2021-acs-fall-national-meeting/ - Oral contribution “Probing the mechanical properties of synthetic and biogenic nanosized lipid vesicles with a plasmon based nanoruler” by Debora Berti.
- ECIS2021, Athens, Greece, 5-10 Sep 2021 (https://www.ecis2021.org/) - Keynote contribution “Assessing the stiffness of synthetic and natural nanovesicles by a “plasmon-based nanoruler”” by Lucrezia Caselli.
- 13th EBSA Congress, Vienna, Austria, 24-28 July (http://ebsa.org/portal/node/332) - Poster contribution “The response of biogenic lipid vesicles to deformations: how pressure and membrane elasticity affect stiffness” by Andrea Ridolfi
New fundamental contribution to the mechanical properties of different lipid phases have been achieved by researchers within the evFOUNDRY consortium. Two preprints are now available on bioRχiv.
The first one (https://www.biorxiv.org/content/10.1101/2021.06.17.448831v1 deals with the mechanical response of lipid vesicles and Supported Lipid Bilayers (SLBs) to indentation and helps understanding how membrane rigidity and internal pressure contribute to the general response of a vesicle to deformations.
The second one https://www.biorxiv.org/content/10.1101/2021.03.29.437497v1 presents the first mechanical characterization of cubic membranes at the nanoscale and reveals an important relationship between the mechanical response of these architectures and their topology.
Andrea Ridolfi contributed to the 10th Annual Meeting ISEV 2021, giving an oral presentation entitled: "AFM-based high throughput mechanical characterization of single EVs from different natural sources". He also won the “Best Interpretative Image” award of the “Image competition" with the image "EV night by Atomic Force Microscopy".
evFOUNDRY contributed to the 10th edition of the International Society for Extracellular Vesicles annual meeting (ISEV2021) https://na.eventscloud.com/website/16865/ with a massive participation. Researchers contributed with:
1. Two oral presentations
a) "Set-up of multi-parametric analytical assays for quantification, quality control and surrogate potency testing of helminth EVs" (Francesca Loria, co-authored by Hansabiomed Life Sciences, University of Aarhus, CSGI-University of Brescia)
b) "AFM-based high throughput mechanical characterization of single EVs from different natural sources" (Andrea Ridolfi, authored by CNR-ISMN, CSGI-University of Florence, CSGI-University of Brescia, University of Aarhus, University of Utrecht, Hansabiomed Life Sciences);
2. One oral poster:
"Pre-processing of bovine milk to clear casein micelles can influence the colloidal and functional properties of milk extracellular vesicles" (from evFOUNDRY project, PhD. Martijn van Herwijnen, co-authored by University of Utrecht, CNR-ISMN, CSGI-University of Florence, CSGI-University of Brescia).
This year, our partner Annalisa Radeghieri chaired the “hot-topic” oral sessions titled “Bone Repair”.
We are proud to announce that Andrea Ridolfi from CNR-ISMN, CSGI-University of Florence won the “Best Interpretative Image” award of the “Image competition. See related news for more details.
A new publication by the Wauben group from Utrecht University on the characterization of bovine milk extracellular vesicles was published in the Journal of Nutrition! The authors show that milk EVs from pasteurized or Ultra Heat Treated (UHT) milk have altered characteristics compared to milk EVs from raw cows milk.
In the latest publication by our evFOUNDRY members from Utrecht, the authors show that extracellular vesicles from human milk support the oral cavity epithelial barrier and modulate both innate and adaptive immune responses. This work was published in the prestigious Journal of Extracellular Vesicles. Additionally, Martijn van Herwijnen and Marca Wauben were invited to the present their work in the EV-Journal Club of the International Society of Extracellular Vesicles (ISEV).
This open assess paper can be accessed via: Journal of Extracellular Vesicles
The recording of the EV-Journal Club can be found on YouTube: EV-Journal Club
The Aarhus group members of evFOUNDRY held a 3 day long PhD course ‘Extracellular Vesicles (EVs) – an introduction’ at Aarhus University, Denmark, 16-18 March 2021. Due to the pandemic, the course was held online. The participants learned the basics of EVs, experimental considerations and functional and translational aspects of the EVs. The course was structured with lectures, group projects, and online practical work.
On 28th-29th October 2021 at the University of Brescia (Brescia, Italy) and virtually, it will take place the WORKSHOP on massive production of EVs Co-organized with the FETopen projects evFOUNDRY and VES4US.
Application & Abstract submission opens April 15th 2021 and closes June 1st 2021. This workshop will be free for accepted applicants and limited to 50 attendees.
On February 1st and 2nd 2021 the evFOUNDRY team met in telematic mode to review the latest 3 months of activity and plan next moves. The meeting saw the participation of all the project researchers in two days of intense discussion. It helped to cheer up a bit from the pandemic difficulties and restrictions. The meeting began with the welcome to the new Project Officer Athina Zampara, who met “in person” the evFOUNDRY team for the first time.
evFOUNDRY researchers Paolo Bergese and Costanza Montis co-edited, together with Kimberly Hamad Schifferili (UMASS - US) a special issue in Biochimica et Biophysica Acta (BBA) - General Subjects on “Biogenic, Hybrid and Synthetic Vesicles”. The full issue is available at the following link:
Check it out!
At the International Society of Extracellular Vesicles’ Infectious Diseases Meeting 2021 on the 25th-28th of January, Anne Borup of the Aarhus-group presented a 10-min talk entitled “Comparison of three enrichment methods for immunomodulatory extracellular vesicles from helminths”. The talk highlighted the findings of the evFOUNDRY work regarding the helminth EVs.
On 23rd November 2020 the evFOUNDRY team met in telematic mode to review the 3 months of activity and plan next moves. The meeting saw the participation of researchers from each partner evFOUNDRY consortium (in particular all the of the young scientists hired on the grant were present) involved in one day of intense discussion (see picture)
By combining DLD with electric fields (eDLD), Prof. Tegenfeldt's group show separation of a variety of nano and micro-sized particles primarily by their zeta potential – which is correlated to their surface charge.
The separation of nanoscale liposomes of different lipid compositions was shown as a proof of principle of this method, with strong relevance for biomedicine and for EVs separation.
Ongoing work focuses on using this type of device to separate EVs based on size and surface charge.
The paper has been labelled as "Feature Paper", "Editor's Choice", and is currently at the top of the "Highly Accessed Articles" list of the journal."
Repository link: Zenodo
Patrizia Andreozzi recently joined the evFOUNDRY team in Florence, Italy. She builded up her strong background on Physical Chemistry of Colloid Systems, in particular in synthesis and characterization of nanomaterials beginning her carrier with a master thesis and PhD in Chemistry at The Sapienza University, Rome. She worked as Post-Doc at Department of Chemical Sciences and Technologies of University of Rome Tor Vergata, in Rome (Italy), at National Nanotechnology Laboratory (NNL) - Nanochemistry Division, Istituto di Nanoscienze del CNR, Lecce (Italy) and at IRCCS Carlo Besta Neurological Institute Fundation, IFOM-Fondazione Istituto FIRC di Oncologia Molecolare, Department of Nanomedicine, in Milan (Italy). From 2016 to 2019 she was Researcher at Centro de Investigación Cooperativa en Biomateriales - CIC biomaGUNE, Department of Soft Matter Nanotecnology, in San Sebastian (Spain).
Finally on November 2020 she joined as Post-doc the evFOUNDRY Team: Welcome on board Patrizia!
Martijn van Herwijnen (Utrecht University) gave a virtual talk entitled “Solving the milk EV puzzle” for the Student Network on EVs (SNEV). SNEV is a virtual society run by students who are interested in the EV field. Its mission is to connect EV research students internationally, to promote discussion, share knowledge, network, and establish collaboration with other students and early career researchers on the extracellular vesicles field through series of virtual events.
Researchers from CSGI in collaboration with Dr. Anna C. Berardi from Unit of Immunohematology and Transfusion Medicine, Spirito Santo Hospital, Pescara, Italy, published a chapter entitled “Extracellular vesicles in regenerative medicine” in the book “Nanomaterials for Theranostics and Tissue Engineering: Techniques, Trends and Applications”. Micro and Nano Technologies series, edited by Filippo Rossi and Alberto Rainer. The chapter gives a concise introduction of the two leading techniques in regenerative medicine: cell-based therapies and tissue engineering. It provides an overview of the most promising non-EV cell-free therapies and discuss EV biological and physicochemical properties and then cover their potential role in regenerative medicine according to the state-of-the-art literature.
Prof. Tegenfeldt’s group (Lund University, Sweden) won the “Best Flash Presentation” award at the 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020; 4-9 October 2020). The 1-minute video has attracted a large crowd of audience to the 1-hour live zoom room where Bao Ho presented the poster entitled “High Throughput Extracellular Vesicle Sorting Using Electrokinetic Deterministic Lateral Displacement” (see picture).
Check the video out, you won’t regret it!!!
evFOUNDRY researchers recently published a paper in which they describe a novel Atomic Force Microscopy (AFM)-based methodology for the simultaneous nanomechanical and morphological analysis of several hundred individual nanosized EVs within the hour time scale. This methodology only requires basic AFM instrumentation and skills for its experimental part, and freely available software for data analysis. In the paper, authors demonstrate how their AFM-based method can be used to quickly detect EV samples contamination by non vesicular aggregates, and to quickly attest the presence of EVs even in samples for which no established assays and/or commercial kits are available, thus making it a valuable tool for the rapid assessment of EV samples during the development of isolation/enrichment protocols by EV researchers.
evFOUNDRY will participate by its coordinator to the session entitled "Voices from the future: Pathfinder stories, people, visions", organized on Sept. 22nd, 09:45 - 12:30 CETA in the frame of the Future Tech Week. The session will be opened by a keynote speech from the neuroscientist Nobel Laureate Professor Edvard I. Moser and followed by a roundtable on the topic of extracellular vesicles involving the coordinators of the cluster of the seven EU-funded ongoing FET projects on extracellular vesicles. Participation is telematic and free, follow the link below to register.
evFOUNDRY has been selected by Innovation Radar as a key innovator project thanks to the “Microfluidic device for EVs extraction” and “Scalable production and SOPs for quality standard nanosized EVs” actions. Innovation Radar is a European Commission initiative to identify high potential innovations and innovators in EU-funded research. It supports projects by suggesting a range of targeted actions to assist them in fulfilling their potential in the market. Both evFOUNDRY actions obtained the following scores: Market maturity: Business Ready Market creation potential: High Innovation Topic: Excellent Science.
More info at: https://www.innoradar.eu/resultbykeyword/evfoundry
On Wednesday 22 July 2020, Andrea Zendrini has been recognized for an Outstanding Oral Abstract Award at the ISEV2020 Virtual Meeting for his abstract The CONAN assay: purity grade and concentration of EV microliter formulations by colloidal nanoplasmonics. His abstract spun off our first collective research paper, which presents key implementations of the COlloidal NANoplasmonic (CONAN) assay developed within the project. It was selected, with other two, among the about 150 oral abstracts featured at the Meeting.
Andrea is a post-doc researcher at Paolo Bergese's group (Università degli Studi di Brescia and CSGI) and at Paolo Ajmone's group (Università Cattolica del Sacro Cuore di Piacenza).
Researchers from Aarhus and Lund University, and in collaboration with Aalborg University, have published a paper in Biomedicines. Here they labelled extracellular vesicles by utilizing parasitic helminths inability to synthesize lipids by culturing helminths with fluorescent lipids. These were taken up by the parasite and subsequently released incorporated into the extracellular vesicles. The released extracellular vesicles were characterized with proteomics and Cryo-TEM and uptake was shown in a human cell line. As the inability of synthesizing lipids is general in parasites, it is believed that this novel methods can be used for tracking extracellular vesicles in the field of parasite-host communication.
evFOUNDRY team enters in COVID-19 lockdown phase 3 with a breath of optimism: Brescia and Aarhus units confirm their collaboration with a student exchange. Research teams of Università degli Studi di Brescia (Department of Molecular and Translational Medicine) and Aarhus University (Department of Clinical Medicine, Infectious Disease), led by Prof. Paolo Bergese and Prof. Peter Nejsum, respectively, will be even more connected thanks to the future biotechnologist Giorgio. Training in Nejsum’s lab will complete his formative internship as well as produce substantial part of the experimental results for his thesis project, being a unique possibility with a strong curricular impact. Nejsum’s lab will also benefit of his visit, as he will bring the expertise and know-how learned during the part of his formative internship already attended in Bergese’s lab.
Researchers from CSGI published a paper on Journal of Microscopy. In this work, they explored the interaction of gold nanoparticles (AuNPs) with synthetic lipid rafts, formed on Supported Lipid Bilayers. Atomic Force Microscopy (AFM) and Neutron Reflectivity measurements (performed at the Heinz Maier-Leibnitz Zentrum, MLZ) were used to characterize the lipid bilayers and to image the AuNPs adsorbed on them. The thickness mismatch present along the rafts' boundaries is thought to generate energetically favourable conditions for the adsorption of NPs, but a direct proof of this mechanism was still missing to date. In the manuscript, AFM images clearly show that AuNPs preferentially target raft's edges when adsorbing on the bilayer.
In a just released research paper, Marca Wauben and coworkers show the inhibitory effects of human milk EVs on T cell proliferation and cytokine release, as well as the inhibition of TLR-triggering on epithelial cells. This hints to a potential nasal/oral application to dampen overactive immune responses, e.g. during the immunopathological responses in lung (and possibly also in gut) reported during SARS-CoV infection.
Researchers from CSGI (Department of Chemistry, University of Florence, Department of Translational Medicine, University of Brescia, CNR Bologna, Italy) made a cover in the journal
Journal of Colloid and Interface Science, with their study on the interaction between superparamagnetic iron oxide nanoparticles and biogenic supported lipid bilayers (SLBs) formed through vesicle fusion of Extracellular Vesicles.
The experimental results highlight a strong interaction of nanoparticles with biogenic SLBs. This evidence strongly suports the hypotheses on the peculiar structure of biogenic SLBs, with cushioned non-flat areas and extended exposed surface; in addition, it suggests that these features are relevant in the response of biogenic membranes to nano-objects.
· Cover picture in Journal of Colloid and Interface Science (see picture). It is an Inside Front Cover.
evFOUNDRY researchers, in collaboration with Stefania Federici and Ivano Alessandri from the Dept. of Mechanical and Industrial Engineering and Dept. of Information Engineering (University of Brescia), published a paper on Journal of Extracellular Vesicles. EV subtype classification is an emerging topic and authors challenged FT-IR spectroscopy to fingerprint EV subpopulations separated using differential centrifugation steps. Large, medium and small EVs from murine prostate cancer (TRAMP-C2) and murine skin melanoma (B16) cell culture media were enriched according to their different sedimentation properties. The combination of FT-IR spectroscopy and spectral exploration with chemometric tools (Principal Component Analysis) were able to fingerprint the EV subpopulations both vertically (deriving from the same cell line) and horizontally (among different cell lines). This work revealed the versatility of this collective approach that can be applied for grading the intrinsic high level of heterogeneity of different EV subtypes.
The CSGI evFOUNDRY team has joined efforts with a multidisciplinary group of researchers from ESRF (Grenoble, France) and ILL (Grenoble, France) to investigate the mechanistic details of the interaction of gold nanoparticles, coated by citrate anion, with synthetic liposomes. The results of this fundamental study will appear in Journal of Colloid and Interface Science.
Thanks to an ensemble of optical, structural and surface techniques, we identified the citrate-lipid ligand exchange at the biomimetic interface as the molecular origin of cooperative behavior, which leads to the formation of clusters of gold nanoparticles on the bilayer. Although this study has a fundamental nature, the implications of membrane-induced clusterization of nanoparticles can be extended to biogenic membranes, as in the case of extracellular vesicles.
Anne Borup, a member of the Aarhus group of evFOUNDRY, has been volunteering at the Aarhus University Hospital during the COVID-19 pandemic. Here a drive-in Corona-test system has been set up in order to ensure safety, minimal spread and test of people suspected to be infected with the Coronavirus. Of high priority is testing of health care professionals with a critical function so they could return to their much-needed services. Anne volunteered at the call center where they received calls from health care professionals that needed to be tested for Corona and subsequently evaluate and organize them for a test in the drive-in.
Ric Gillams and Hywel Morgan, evFOUNDRY's researchers from the University of Southampton have developed a prototype personal respirator called ‘PeRSo’, intended to address the limitations of the existing protective equipment doctors and nurses are wearing on the wards. Our prototype consists of a fabric hood which covers the wearer’s head, and a plastic visor to protect their face. A small portable unit delivers clean air through a High Efficiency Particulate Air (HEPA) filter to the wearer from a battery-powered fan pack mounted on a belt. In a rapid development process we have gone from a problem specification to working prototypes within a week, and conducted preliminary tests.
Ric Gillams described the PerSo to BBC South Today and Times News.
evFOUNDRY researchers in collaboration with Andrea Zendrini - to date post-doc researcher at Università degli Studi di Brescia and at Università Cattolica di Piacenza - published a protocol that describes how to assign purity grade and titrate extracellular vesicle solutions of a few microliters. The assay is an augmented version of the COlorimetric NANoplasmonic (CONAN) assay developed in 2015 by Paolo Bergese's group. It has been implemented in the first year of activity of evFOUNDRY, being now part of the standard operating procedures (SOPs) for preparing quality standard nanosized EVs from bovine milk and Ascaris media.
The Aarhus group members of evFOUNDRY held a 3 day long Ph.D. course ‘Extracellular Vesicles (EVs) – an introduction’ at Aarhus University,
Denmark, March 2020. The participants learned the basics of EVs, experimental considerations and functional and translational aspects of the EVs. The course was structured with lectures, group projects, and laboratory work.
On February 4th-6th 2020, the evFOUNDRY team met in Aarhus (hosted by Aarhus University - https://pure.au.dk/portal/en/persons/peter-nejsum(ee468e32-0a8b-4cd3-9473-60335cf27df9).html) to review the first 18 months of activity and plan next moves. The meeting saw the participation of researchers from each partner of the evFOUNDRY consortium (see picture) involved in three days of intense discussion.
During this special occasion, two ESAB members (Andrew Hill https://www.andyfhill.com/ and Pierre Joseph https://www.laas.fr/public/en/mile) attended an evFOUNDRY meeting for the first time.
On 4thFebruary the Workshop on "knowledge management and IPR" was organized within the collaboration with the team of FET-Briefing project (fetbriefing.eu). The Workshop was organized in a telematic mode, through the direct connection from Aarhus to the live event held in Stuttgart, Germany.
Aarhus University is offering a 3-day PhD course with the title “Extracellular Vesicles – an introduction” in March 2020.
More details can be found here:
To celebrate the 15th Anniversary of Soft Matter Costanza Montis (evFOUNDRY young researcher) and Debora Berti have been chosen to write their personal perspectives on what are the hot topics in their area, and where they think their sub-field is going. Their contribution reviews the state of art on hybrid soft matter assemblies composed of inorganic nanoparticles (NP) and lamellar or non-lamellar lipid bilayers and the the latest developments on the use of lipid bilayers as coating agents for inorganic NPs are presented. Costanza Montis finally acknowledges evFOUNDRY Project.
On 11-13 December 2019 Andrea Ridolfi (CSGI-Bologna) partecipated with a poster entitled “Nanomechanical properties of synthetic and natural lipid bilayers through Atomic Force Microscopy (AFM): from Supported Lipid Bilayers (SLBs) to vesicles” to the Workshop “Bill2019” Grenoble, France.
evFOUNDRY researchers of the University of Brescia and CSGI explain on the first 2020 issue of Biomaterials Science how scientists are learning to exploit the wealth of nanoparticles that populate the secretome, such as extracellular vesicles and lipoproteins. The review article has been also featured in the issue back cover.
On the 10rd of December 2019, the Southampton group of evFOUNDRY participated in the UK EV forum, hosted by The UK Society for Extracellular Vesicles (UKEV) in London, UK. Ric Gillams presented a poster and flash talk showing results related to evFOUNDRY (see picture).
Paolo Bergese (evFOUNDRY coordinator) and Marca Wauben (Utrecht University partner) contributed to set the ISEV position paper on biological membranes in EV biogenesis, stability, uptake, and cargo transfer. The paper assesses current knowledge of several aspects which are key to evFOUNDRY, including EV structural and colloidal properties at the individual level and in relation to their interaction with surfaces and nanomaterials.
The 1ST CLUSTERING EVENT FOR EXTRACELLULAR VESICLES FET-OPEN PROJECTS, held in Palermo in November 06th 2019, involved the 5 EU funded ongoing FET OPEN projects on EVs, counting participation of 34 research and industrial organizations from 15 EU countries. The first collective action of the newborn cluster was producing the attached position document, the evMANIFESTO, which aims at highlighting the current EU effort in EV research within the FET program and the urgency of introducing and securing more firmly the EV-related theme as a cornerstone in the research agenda in EU.
You can find the document in the results section:
evFOUNDRY sponsored a special session on Extracellular Vesicles (EVs) within the workshop "Science through Scanning Probe Microscopy 2019" StSPM19, held at the National Research Council of Bologna on November 21st and 22nd 2019. The session was focused on the study of EVs through Atomic Force Microscopy; among the speakers: Paolo Bergese and Andrea Ridolfi for evFOUNDRY, Gijs Wuite (VU University, Amsterdam) and Pietro Parisse (Elettra-Synchrotron, Trieste) as external contributions.
evFOUNDRY members organized and participated at the 1st symposium of the Italian Society of Extracellular Vesicles held in Palermo from the 6th to the 8th of November 2019. Annalisa Radeghieri (CSGI-Brescia) organized the meeting as part of EVIta directive board. Paolo Bergese (CSGI-Brescia) was selected for oral presentation "Projecting extracellular vesicles in two dimension" as well as Marco Brucale (CSGI-Bologna) "Nanomechanics of individual extracellular vesicles". Lucia Paolini (CSGI-Brescia) presented a poster. Francesco Valle (CSGI-Bologna), Natasa Zarovni (Hansa-Biomed) and Francesca Loria (Hansa-Biomed) participated at the symposium. Peter Nejsum (Aarus University) gave a morning lecture on “A new level of complexity in host-parasite interaction- Extracellular Vesicles“
On 6th of November 2019 in Palermo (Italy) evFOUNDRY was actively involved together with 4 ongoing FET projects: INDEX, VES4US, GLADIATOR and MINDGAP in the 1st Clustering Event for EVs FET-OPEN projects. 34 organizations from 15 countries were present to the event during which the EVs network has taken off. A letter of Intent to support EVs research was signed and an EVs Manifesto will be created.
Lucia Paolini was selected for a flash presentation at the 1st Lugano ExoDay on: “EV subpopulations fingerprinting by Fourier-transform Infrared (FT-IR) spectroscopy”.
The workshop was organized the 25th of October by several Swiss research institutes: University of Southern Switzerland, Cardiocentro Ticino Foundation, Ente Ospedaliero Cantonale.
Researchers from Tegenfeldt group, Lund University, Sweden (Jason Beech, Bao Ho, Jonas Tegenfeldt) and Guiducci group, EPFL, Switzerland (Kevin Keim, Carlotta Guiducci) made a cover in the journal Advanced Materials Technologies with their novel metal-coated Electrokinetic Deterministic Lateral Displacement (Electrokinetic DLD) devices. These devices bring about several advantages: tuneable particle separations, significant reduction the critical size, and increase in the dynamic range with switching times on the order of seconds. Most notably, in a device having gap size of around 10 µm, polystyrene particles as small as 250 nm can be displaced, demonstrating two-orders of magnitude reduction in critical size using Electrokinetic DLD, as compared to conventional DLD.
An evFOUNDRY presentation starred the FET seminar series on Thursday October 10th at the European Commission Covent Garden building (Brussels). The seminar was actively participated by a varied audience, including EU scientific project officers, economists and policy makers.
On the 11th of October 2019 all members of evFOUNDRY Consortium met at Research Executive Agency in Brussels for the first Reporting Period Review Meeting.
Our project officer, Adriana Godeanu-Metz, chaired the meeting sessions.
All the activities conducted during in the different work packages have been presented and discussed with the reviewers.
On the 3rd of October 2019, the Aarhus group of evFOUNDRY participated in the 1st annual meeting in The Danish Society of Extracellular Vesicles (DSEV) held in Aarhus, Denmark. Peter Nejsum and Anne Borup are on the board and founders of the society and were responsible for organizing the event. Anne presented a poster of results related to evFOUNDRY (see picture).
On September 27th evFOUNDRY took part at the MEETmeTONIGHT (European Researchers’ Night) and Future Tech Week events.
CSGI members introduced students, kids and general public to evFOUNDRY aims and strategies with a ludic-scientific stand. Venue of the event has been Santa Giulia museum in Brescia, Italy.
Debora Berti has been appointed as SAB member for Swedish Drug Delivery Center for the period 2020-2024 with a budget of 3M Euro.
On September 5-6 2019, the evFOUNDRY team met in Bologna (hosted by ISMN – CNR - www.ismn.cnr.it) to review the first 12 months of activity and plan next moves also in the perspective of the review meeting that will be held in Bruxelles in October. The meeting saw the participation of researchers from each partner of the evFOUNDRY consortium (see picture) involved in two days of intense discussion.
On the 4th of September, a workshop was held in Brescia, Italy for members of the evFOUNDRY project. Bao Dang Ho (Lund, Sweden) and Francesca Loria (Estonia, Hansa-Biomed) attended a theoretical and practical course on Colorimetric Nanoplasmonic (CONAN) assay developed in Paolo Bergese’s lab.
On the picture, from the left: Lucia Paolini, Bao Dang Ho and Francesca Loria.
On 30 July 2019 Paolo Bergese introduced evFOUNDRY at the National University of Singapore (NUS), while giving a research seminar on "Nanotechnology of Cell Communication". The seminar was hosted by Prof. Rachel Ee (Dep. of Pharmacy, Faculty od Science) and Prof. James Kah (Dep. of Biomedical Engineering, Faculty of Engineering).
Joy Wolfram, Sara Busatto, Mauro Ferrari and Paolo Bergese contributed, together with other international bio-nano experts, to comment the paper "Minimum information reporting in bio–nano experimental literature - MIRIBEL" . The correpondence is now published by Nature Nanotechnology. Their piece advocates integrating MIRIBEL (which is focused on synthetic nanoparticles) with specific considerations related to biogenic nanoparticles such as extracellular vesicles.
evFoundry members attended the XLVII National meeting of the Physical Chemistry Division of the Italian Chemistry Society (Physics Department, "Sapienza" University of Rome, 1-4 July 2019) with several contributions:
Costanza Montis with a talk entitled "Interaction of inorganic nanoparticles with synthetic and biogenic lipid membranes: fundamental understanding and biomedical applications'"
Andrea Ridolfi with a poster: "Nanomechanical classification of extracellular vesicles" (see figure)
Debora Berti with a talk entitled "Nanostructured materials interacting with synthetic and natural lipid mesophases: challenges and opportunities"
See the meeting website for more details: http://congressodcf2019.it/english/
Costanza Montis gave a talk entitled "Interaction of inorganic nanoparticles with synthetic and biogenic lipid membranes: fundamental understanding and biomedical applications" in the Young Investigator Perspective section at the 9th International Colloids Conference, Sitges (Barcellona, Spain), June 16-19 2019.
See the meeting website for more details: https://www.elsevier.com/events/conferences/international-colloids-conference
From May 27 to May 29, Utrecht University was the stage for a workshop of milk EV isolation. In the group of Marca Wauben, visitors Richard Gillams (Southampton, UK) and Bao Dang Ho (Lund, Sweden) experienced first-handedly what its like to process raw cow's milk and to prepare size exclusion columns and density gradients needed for further purification of milk EVs.
Congratulations Ric and Bao, for your first batch of purified milk EVs!
The International Society for Extracellular Vesicles (ISEV) is pleased to announce two Special Achievement Awards that were presented at the ISEV2019 annual meeting in Kyoto, Japan, 24–28 April 2019. ISEV Special Achievement Awards are given each year to one or more individuals who have made outstanding contributions to the field of extra- cellular vesicle (EV) research or have performed extraor- dinary service to ISEV. In 2019, ISEV Special Achievement Awards were presented to Professors Takahiro Ochiya, PhD, and Marca Wauben, PhD (see Figure)
On the 24th of May, a workshop was held in Aarhus, Denmark for members of the evFOUNDRY. It was a full day of discussion and demonstration of how Peter Nejsum’s group isolate and characterize EVs from Ascaris Suum helminths. Richard Gillams (Southhampton, UK) and Bao Dang Ho (Lund, Sweden) participated.
On the picture, from the left Richard Gilliams, Anne Borup and Bao Dang Ho.
evFOUNDRY will be at the annual meeting of the International Society of Extracellular Vesicles (ISEV) (Kyoto from 24th-28th April 2019), contributing to several sessions:
Martijn van Herwijnen (Utrecht University) will give a lecture on “Therapeutic Potential of Milk EVs” at the educational day (24th April)
Annalisa Radeghieri (CSGI) will give an oral presentation entitled “Different ATT isoforms are associated to EVs from ATT type II deficient patients” at the “EVs in Blood and Blood Disorders” session (25th April)
Lucia Paolini and Martijn van Herwijnen will present a poster on “The extracellular vesicle foundry: evFOUNDRY” at the “Network/society” session and will attend the ISEV Board (26th April)
Marca Wauben (Utrecht University, Secretary General of ISEV and part of the Organizing Committee of ISEV2019) will chair the “Plenary Session on RNA” (27th April).
Lucia Paolini will present two posters: “Fourier-transform Infrared Spectroscopy (FT-IR) to fingerprint EV subpopulations as a whole” and “Taxonomy of individual EVs by nanomechanics” at the “Advances in EV Quantification and Characterization” poster session (27th April).
See the meeting program for more details: https://www.isev.org/page/ISEV2019
On Thursday April 11 Lucia Paolini (evFOUNDRY personnel, CSGI) gave an invited talk at the “Mini-Workshop on Extracellular Vesicles: from biophysical properties to therapeutic applications” within EXOTHERA project funded by Interreg Italia-Österreich programme. The talk presented “evFOUNDRY: the extracellular vesicle foundry” project (see flayer for details).
First, she was awarded ‘best poster presentation’ at the PhD day, 2019 at Aarhus University and secondly she was awarded the ‘young scientist award’ for best poster presentation at the Spring Symposium 2019 in Copenhagen hosted by the Danish Society for Parasitology.
On March 27th-29th 2019, the evFOUNDRY team met in Lund (hosted by LINXS - Lund Institute of Advanced Neutron and X-ray Studies http://www.linxs.lu.se) to review the first 6 months of activity and plan next moves. The meeting saw for the first time the participation of all the involved young researchers (see picture, from the left Karolina Mothander (Sweden), Lucia Paolini (Italy), Richard Gillams (UK), Andrea Ridolfi (Italy), Anne Borup (Denmark), Anders T Boysen (Denmark), Costanza Montis (Italy), Francesca Loria (Estonia), Bao Dang Ho (Sweden), Martijn van Herwijnen (The Netherlands) not in the picture).
The faculty of Veterinary Medicine from Utrecht University has released a new volume of VetScience magazine. This special issue on Future Food includes interviews with Professor of Intercellular Communication Marca Wauben and Martijn van Herwijnen, who explains about his work about milk-derived Extracellular Vesicles (in Dutch). http://www.uu.nl/sites/default/files/vetscience_nov2018.pdf
Members of the Evfoundry consortium have taken the initiative, and for the first time, been running the 3 day long Ph.D. course 'Extracellular vesicles (EVs) – an introduction' at Aarhus University, Denmark, March 2019. There were 10 participants from different universities in Denmark. The participants were introduced to EVs and different aspects of EV biology and how to characterize and explore the function of EVs. During the laboratory exercises the participants enriched for EVs and characterized their sizes and numbers and finally, used western blots to identify specific markers of EVs.
Annalisa Radeghieri (evFOUNDRY personnel) has received 120,000 euro (approximately USD 137,000) in funding in the framework of an Italian Education and Research Ministry (MIUR-PRIN) grant. The project aims at “Development of biotechnological nanoparticle platform for the delivery of antitumor therapies using Patient Derived-Organoids library of Breast cancer”. It brings in collaboration University of Milano-Bicocca, University of Milan, National Research Council and University of Rome Tor Vergata.
Danish Professor Peter Nejsum (evFOUNDRY personnel) has received DKK 3 million (approximately USD 457,800) in funding for a project to develop a new method to diagnose and treat diseases. The aim is to discover new immunotherapies and diagnose cancer through blood tests. But first step on the journey goes to the local abattoir to collect a bucket of roundworm.
Sara Busatto and Paolo Bergese (evFOUNDRY PI) co-authored with Joy Wolfram and colleagues from the Mayo Clinic and the Houston Methodist Research Institute a paper on the use of tangential flow filtration (TFF) for highly efficient concentration of nanosized EVs from large volumes of fluid. The study contributes to evFOUNDRY effort in exploring methods for robust massive separation of EVs based on size.
Debora Berti, member of the CSGI team has been appointed in the Editorial Board of Advances in Colloid and Interface Science, an international journal devoted to experimental and theoretical developments in Interfacial and Colloidal Phenomena and their implications in Biology, Chemistry, Physics and Technology. https://www.journals.elsevier.com/advances-in-colloid-and-interface-science/editorial-board
Researchers involved in evFOUNDRY contributed to the last update of the “Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines”, which was published online in the Journal of Extracellular Vesicles at the beginning of this week. https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1535750
evFOUNDRY researcher Lucia Paolini was selected for the FET2RIN training course (three workshops from mid-December 2018 to mid-February 2019 in Brussels).
The training, in collaboration with META, European Crowdfunding Network (ECN), and European Business and Innovation Centre Network (EBN), will help to look at FET project from a business perspective, assessing its strengths and weaknesses on the route to commercialization (http://www.fet2rin.com/home).
KICK OFF PARTNERS’ MEETING On September 12 2018, at Plesso di SANTA TERESA Florence, next to the workshop (https://www.quotidiano.net/blog/malpelo/ricerca-vescicole-extracellulari-come-taxi-per-farmaci-e-vaccini-33.2061 http://www.meetthelifesciences.it/it/archvio/831-nanotecnologie-evfoundry-nuovi-approcci-ed-utilizzi-delle-vescicole-extracellulari) it’s time for the partners meeting.
On October 5 2018, Lucia Paolini (evFOUNDRY recruited researcher) with Annalisa Radeghieri (evFOUNDRY personnel, CSGI), Natasa Zarovni (evFOUNDRY personnel, HansaBioMed Life Sciences) and Paolo Bergese (evFOUNDRY coordinator) will participate in the foundation of the Italian Society of Extracellular Vesicles - EVITA. The meeting will be held at CNR headquarters in Rome. EVITA will connect leading Italian scientists from all the disciplines involved in EV research in the effort to advance EV research nationally and globally, in tight relationship with other EV Societies, including The International Society for Extracellular Vesicles (https://www.isev.org), the Spanish GEIVEX (http://geivex.org) and the American Society for Exosomes and Microvesicles (https://www.asemv.org).
On Friday September 21 Annalisa Radeghieri (evFOUNDRY personnel, CSGI) will give an invited talk at the International Conference on Bioengineering and Cell Therapy.
The talk will be focused on the potentiality of extracellular vesicles in regenerative medicine.
On September 12 2018, at Plesso di SANTA TERESA Florence , evFOUNDRY kicks off and opens to academic and non-academic communities with a free workshop on “Extracellular vesicles, a breakthrough in precision medicine and nanotechnology”, which sees the partners lecturing on their role and science in the project.
Vescicole extracellulari come navette, consegnano farmaci e vaccini. https://www.quotidiano.net/blog/malpelo/ricerca-vescicole-extracellulari-come-taxi-per-farmaci-e-vaccini-33.2061 .
Nanotecnologie: nuovi approcci ed utilizzi delle vescicole extracellulari. http://www.meetthelifesciences.it/it/archvio/831-nanotecnologie-evfoundry-nuovi-approcci-ed-utilizzi-delle-vescicole-extracellulari
CSGI, Department of Chemistry University of Florence,
via della Lastruccia 3, 50019 Florence (Italy)
CSGI, Department of Molecular and Translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy
CSGI, Department of Chemistry University of Florence, via della Lastruccia 3, 50019 Florence (Italy)
evFOUNDRY has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 801367